Fig. 6: Subgenomic nucleocapsid RNA, ORF1a, and PFU quantification at early stages of infection. | Nature Communications

Fig. 6: Subgenomic nucleocapsid RNA, ORF1a, and PFU quantification at early stages of infection.

From: Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5

Fig. 6

a Calu-3 and b Vero B4 cells were infected at an MOI of 0.002 with one virus isolate of each phylogenetic lineage in biological duplicates for each indicated time point in n = 2 independent experiments. At the indicated time points, cell culture supernatant was harvested and subjected to virus quantification by plaque assay. Infected cells were lysed and total RNA was isolated and subjected to quantification by q-RT-PCR of sgmRNA N and ORF1a RNA, normalized to the housekeeping gene TBP, and the amount of RNA at 0 hpi. Shown are the combined data of two independent experiments. Statistical significance of variance in virus replication between groups was analyzed by one-way ANOVA. Significant differences were found for sgmRNA N (in a) Calu-3: **; p = 0.0026; in b) Vero B4: *; p = 0.0276), for ORF1a RNA (in a) Calu-3: **; p = 0.0035; in b Vero B4: *; 0.0418) and for PFU/ml in Calu-3 (*; p = 0.0139) PFU, plaque forming units; sgmRNA N, subgenomic nucleocapsid mRNA; ORF, open reading frame.

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