Fig. 6: NR2F1 mediates the role of NAS1 in dormancy by inhibiting ΔNp63 transcription. | Nature Communications

Fig. 6: NR2F1 mediates the role of NAS1 in dormancy by inhibiting ΔNp63 transcription.

From: Long non-coding RNA NR2F1-AS1 induces breast cancer lung metastatic dormancy by regulating NR2F1 and ΔNp63

Fig. 6

a Expression heatmap of genes affected by NAS1 and NR2F1 in both MCF10CA1h and MCF7. b qPCR verification of ΔNp63 regulation by NAS1 and NR2F1. c qPCR verification of miR-205 regulation by NAS1 and NR2F1. d ΔNp63 protein levels regulated by NAS1 or NR2F1. e, f EMT marker protein expression (e) and tumorsphere formation (f, n = 3 culturing experiments) after ΔNp63 knockdown in CA1h-P2 cells with NAS1 knockdown. g GSEA analyses of the gene set upregulated and downregulated by ΔNp63 in MCF7 cells with NR2F1 overexpression versus control. h ChIP-qPCR analysis of NR2F1 binding to ΔNp63 promoter (n = 3 replicates from one experiment). P1–3, primer pairs #1–3. i mRNA levels of EMT markers in MCF10AT with NAS1 overexpression and/or miR-205 mimic. Data represent mean ± SD. Statistical significance was determined by a two-tailed unpaired t test. Experiments were repeated at least three times independently with similar results; data from one representative experiment are shown. Scale bar, 100 μm.

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