Fig. 4: ROS regulation by FIGs-LC.
From: Peroxisome inspired hybrid enzyme nanogels for chemodynamic and photodynamic therapy
a Schematic ROS regulation of LOx-modulated ·OH and CAT-modulated 1O2 production. b Changes (F/F0) in the fluorescence intensity of O2 probe (Ru(dpp)) over time. c ESR spectra of 1O2 with TEMP as trapper. d ESR spectra of ·OH with DMPO as trapper, red clubs: hydroxyl free radicals; black diamonds: oxidized DMPO radicals. e Degradation (A/A0) of methylene blue (MB) over FIGs, FIGs-L, and FIGs-LC in the simulated TME solutions with pH 6.0 (100 μM of H2O2, 10 mM of GSH, and 2 mM of lactate). f Relative degradation rates of MB according to the iron concentration. g Relative fluorescence intensity of SOSG as a function of laser irradiation time for the FIGs and FIGs-LC. h Fluorescence intensity of SOSG at 10 min for the FIGs and FIGs-LC. i The MB degradation curve of FIGs-LC with the different loading amount of LOx and CAT (1:3, 1:2, 2:1, LOx:CAT, U/U) under the simulated tumor microenvironment. j MB degradation rates of FIGs-LC with different loading ratios between LOx and CAT, and each histogram represents the mean ± s.d. k The fluorescence changes of 1O2 probe (SOSG) of FIGs-LC with different loading amounts of LOx and CAT (1:3, 1:2, 2:1, LOx:CAT, U/U) under the simulated tumor microenvironment. l The 1O2 production of FIGs-LC with different loading ratios between LOx and CAT at 5 min. In k and l, data are presented as mean ± s.d. from three independent replicates; p values were analyzed by Student’s two-sided t-test.
