Fig. 4: Diversity of [FeFe] hydrogenases in Woesearchaeota.

a Phylogenetic analysis of the catalytic domain of [FeFe] hydrogenase sequences (n = 856, trimmed alignment length = 356) based on LG + G + C10 model using IQ-Tree (v1.6.8). Black dots denote nodes with UFBOOT values ≥95%. Scale bar shows the average number of substitutions per site. Woesearchaeota sequences are colored in red embedded in the group A sequences. b Probability plot of the occurrence of each amino acid of the P1, P2, and P3 motif of [FeFe] hydrogenase group A3. Amino acids are colored based on their hydrophobicity score. Gray box is added to distinguish the letters V and I from Y. Positions of alignment are indicated below the amino acids. c Conservation of the P1, P2, and P3 motif of selected amino acid sequences in comparison to Thermotoga maritima (WP_004081677.1), Desulfovibrio fructosivorans (WP_005990603.1), and Clostridium pasteurianum (WP_023973103.1). The difference to the reference sequences is colored by their hydrophobicity score and conserved site is colored in gray. The position of amino acids flanking the P1, P2, and P3 motif in the alignment is indicated at the bottom of the plots. Source data are provided as a Source Data file.