Fig. 5: Cryo-EM maps around Pdr5 ligands.
From: Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5
The panels show the details of the cryo-EM map surrounding (a–d) the nucleotides and (e) rhodamine 6G (cf. Fig. 4 and Supplementary Fig. 10). The domain colouring follows the scheme of the previous figures. a The catalytic site (NBS2) of Pdr5 in inward-facing conformation (ADP-Pdr5) with bound ADP. b The catalytic site of outward-facing Pdr5 (AOV-Pdr5) in a vanadate-trapped state that mimics the intermediate step of hydrolysis with an ADP-orthovanadate molecule. Vanadium is shown as a grey sphere and co-ordinated magnesium ion is depicted as a green sphere. c The deviant, inactive site (NBS1) of Pdr5 in the inward-facing conformation (ADP-Pdr5), showing the bound ATP. d The same site in the outward-facing conformation (AOV-Pdr5).
