Fig. 7: Microglia regulate vascular function through PANX1–P2RY12 coupling.

a–c Representative laser speckle images (a, b) and graphical tracing (c) of cerebral blood flow in control and following PLX3397 treatment. d–f Quantification of basal cerebral blood flow (d), cerebral blood flow in response to CO₂ response (e), and the change in cerebral blood flow with CO₂ treatment (f) in control or PLX3397-treated conditions. n = 10 control and 19 PLX3397-treated mice. g–l Quantification of basal cerebral blood flow (g, n = 5–7 mice each and j, n = 4–5 mice each) cerebral blood flow in response to CO₂ response (h, n = 5–7 mice each and k, n = 4–5 mice each) and the change in cerebral blood flow with CO₂ treatment (i, n = 5–7 mice each and l, n = 4–5 mice each) in P2RY12^+/+ and P2RY12^−/− mice (g–i) and PANX1^+/+ and PANX1^−/− mice (j–l) (n = 4–7 mice each). Data are presented as mean values ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Two-sided unpaired Student’s t test.