Fig. 6: Overexpression of Trappc4 affects tumor microenvironment composition and immunotherapy efficacy.

a Schematic representation of the xenograft mouse model and dosage regimen. Mice bearing control or Trappc4-OE MC38 tumors were treated with anti-PD-L1 mAb. b–d Statistical analysis of tumor volumes (b) (vector+α-PD-L1, n = 13; all other groups n = 14), representative images of tumors (c) and tumor weights (d) (vector + IgG, n = 12, Trappc4-OE + IgG, n = 12, Vector+α-PD-L1, n = 10, Trappc4-OE + α-PD-L1, n = 10) of the mice with subcutaneous tumors in different groups. Values indicate means ±SEM in (b) and means ±SD in (d), compared by nonparametric Mann–Whitney test, two-sided. (^*P = 7.2e-5) e–h The percentages of CD8⁺ T cells (e), CD69⁺ CD8⁺ T cells (f), CD107a⁺ CD8⁺ T cells of CD45⁺ cells (g) and IFN-γ⁺ cells of CD8⁺ T cells (h) in each group (n = 6 per group) were analyzed by flow cytometry. The statistics were plotted as means ±SD, compared by two-sided Student’s t test in e, g, h, and nonparametric Mann–Whitney test in f. OE, overexpression. (^**P = 3.6e-5). Source data are provided as a Source Data file.