Fig. 6: Phosphorylation of nucleosides by uP4 (2) and electropherograms of the CE-MS analysis. | Nature Communications

Fig. 6: Phosphorylation of nucleosides by uP4 (2) and electropherograms of the CE-MS analysis.

From: The chemistry of branched condensed phosphates

Fig. 6

a Conditions and phosphorylation rates for the reaction of nucleosides with uP4. If the pH of the nucleophile solution was adjusted, the applied base is indicated in brackets. Phosphorylation rates were calculated without consideration of the phosphoramidate by-product arising from the reaction of DBU with uP4. b Electropherograms of the CE-MS analysis of the product distribution. The positional isomers were determined by spiking experiments.

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