Fig. 3: TNF-α leads to elevated expression of ELMO1 in AS-MSC. | Nature Communications

Fig. 3: TNF-α leads to elevated expression of ELMO1 in AS-MSC.

From: TNF-α-mediated m6A modification of ELMO1 triggers directional migration of mesenchymal stem cell in ankylosing spondylitis

Fig. 3

a Cluster heatmap of HC-MSC (n = 6) and AS-MSC (n = 6) without and with 100 ng/ml TNF-α treatment. b PCA analysis of HC-MSC (n = 6) and AS-MSC (n = 6) without (W/O) and with 100 ng/ml TNF-α treatment. c Venn diagram of HC-MSC or AS-MSC without (W/O) and with 100 ng/ml TNF-α treatment. d The top 10 significant pathways enriched from the 489 critical genes in KEGG analysis. e Heatmap and fold changes in the 27 upregulated genes and 11 downregulated genes in the chemokine signaling pathway. f ELMO1 mRNA expression in AS-MSC (n = 9) was higher than that in HC-MSC (n = 9) after 100 ng/ml TNF-α treatment. P = 1.075E−8 based on one-way ANOVA followed by Bonferroni’s post hoc comparisons. g ELMO1 expression in AS-MSC (n = 9) was higher than that in HC-MSC (n = 9) after 100 ng/ml TNF-α treatment at the protein level. P = 0.000253 based on two-tailed Student’s t-test. h Immunofluorescence results showing that the fluorescence intensity of ELMO1 was stronger in AS-MSC than in HC-MSC after 100 ng/ml TNF-α treatment. Red fluorescence indicated ELMO1 expression and blue fluorescence indicated cell nucleus. Experiment was repeated nine time independently. Scar bar = 20 μm. i After treatment with 100 ng/ml TNF-α, the active Rac1 level of AS-MSC (n = 9) was higher than that of HC-MSC (n = 9). P = 0.00018 based on two-tailed Student’s t-test. Values are presented as the mean ± SD. *** Indicates P < 0.001. W/O TNF-α means without TNF-α.

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