Fig. 7: Inhibiting METTL14 expression increases ELMO1 expression and promotes MSC directional migration.
a METTL14 expression in MSC was lower in Lv-M14 group (n = 9; P = 7.2995E−14), and ELMO1 expression was higher in Lv-M14 group compared to Lv-NC group (n = 9; P = 1.3076E−13). b The m6A modification level of Lv-M14-transfected MSC (n = 9) was lower compared to that of control MSC (n = 9; P = 3.0324E−11) and Lv-NC-transfected MSC (n = 9; P = 2.7411E−12). c, d The degeneration rate of ELMO1 mRNA in Lv-M14 group (n = 9) was slower than that in control (n = 9; P = 0.00013) and Lv-NC group (n = 9; P = 0.000331). e The Lv-M14 group (n = 9) had higher active Rac1 levels than control group (n = 9; P = 8.9659E−11) and Lv-NC group (n = 9; P = 6.3663E−11). f There were more stained migratory MSC in the Lv-M14 group (n = 9) than in the control group (n = 9; P = 7.6189E−9) and Lv-NC group (n = 9; P = 1.3407E−8). g The migratory area of the Lv-M14 group (n = 9) was larger than that of the control group (n = 9; P = 1.7401E−11) and Lv-NC group (n = 9; P = 7.5404E−12). h The bioluminescence area of the Lv-M14 group (n = 9) was larger than that of the control group (n = 9; P = 4.093E−8) and Lv-NC group (n = 9; P = 2.0632E−7). Data were analyzed using one-way ANOVA followed by Bonferroni’s post hoc comparisons. Values are presented as the mean ± SD. *** Indicates P < 0.001. W/O indicates MSC without lentivirus transfection. Lv-NC indicates MSC transfected with control lentiviruses. Lv-M14 indicates MSC transfected with lentiviruses encoding an shRNA specific for METTL14.
