Fig. 9: METTL14 acts on the specific m6A modification site of the ELMO1 3′UTR. | Nature Communications

Fig. 9: METTL14 acts on the specific m6A modification site of the ELMO1 3′UTR.

From: TNF-α-mediated m6A modification of ELMO1 triggers directional migration of mesenchymal stem cell in ankylosing spondylitis

Fig. 9

a CLIP-PCR assay results showed that ELMO1 expression was much higher in the anti-METTL14 antibody group (n = 9 biologically independent cells) than in the IgG control group (n = 9 biologically independent cells). P = 0.000018 based on two-tailed Student’s t-test. b Luciferase activity was lower in the wild-type METTL14 group (n = 9 biologically independent experiments) compared to the R298P mutant METTL14 group (n = 9 biologically independent experiments; P = 6.5606E−14) and NC group (n = 9 biologically independent experiments; P = 2.26E−13). Data were analyzed using one-way ANOVA followed by Bonferroni’s post hoc comparisons. c The mutated sites (A to T) in the ELMO1 3′UTR. d The luciferase activity of the mutant 1 group (n = 9 biologically independent experiments) was restored to control levels, but the luciferase activity of the mutant 2 (n = 9 biologically independent experiments; P = 1.7615E−18) and 3 groups (n = 9 biologically independent experiments; P = 2.6302E−17) as well as the wild-type ELMO1 group (n = 9 biologically independent experiments; P = 2.1376E−18) were significantly lower. Values are presented as the mean ± SD. *** Indicates P < 0.001 compared to the IgG or NC group.

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