Fig. 2: AtLEA4-5 is highly sensitive to the chemical composition of the solution. | Nature Communications

Fig. 2: AtLEA4-5 is highly sensitive to the chemical composition of the solution.

From: Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells

Fig. 2

a Computational solution space scan of the normalized radius of gyration (Rg) of AtLEA4-5 (blue), five different scrambled sequences shown in Fig. 1e, f (red), and 70 different naturally occurring IDRs (gray) under different solution repulsion levels (low to high solution repulsion of the protein backbone). Mean ± SD from n = 5 independent simulations. For clarity, only AtLEA4-5 (blue) SD is shown. SD for the other proteins are provided in the Source Data file. b Experimental solution space scan of AtLEA4-5 and CS. Open circles show the normalized FRET ratio (DxAm/DxDm) for the indicated concentration of each solute, with two points (that often overlap) for each concentration taken from separate repeats, highlighting the reproducibility of the data. Background color intensity represents sensitivity to the addition of solute. Stronger colors indicate stronger sensitivity. Red: compaction; blue: expansion; white: no change. Solution concentrations are given in weight percent (0–25 or 0–12 wt%) or molar (0–1.5 M). Source data are provided as a Source Data file.

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