Fig. 3: SED1 can dynamically track the effects of osmotic stress on live yeast cells.
a Normalized FRET ratio (DxAm/DxDm) time course of live yeast cells expressing SED1, treated with different concentrations of NaCl. The arrow indicates the addition of the treatment. Mean ± SEM. One-way ANOVA. ***p < 2 ×10−16. b Normalized FRET ratio (DxAm/DxDm) of live yeast cells expressing SED1 (blue) and CS (green), treated with different concentrations of NaCl. One-way ANOVA. ***p < 2 ×10−16. Continuous lines were smoothed using R with a loess smoothing function. Shaded regions indicate 95% confidence intervals. c Normalized FRET ratio (DxAm/DxDm) of wild type BY4742 strain (blue), hog1∆::G418 mutant (gray), and pbs2∆::G418 mutant (black), live yeast cells expressing SED1, hyperosmotically shocked with different concentrations of NaCl. Measurements were done immediately after hyperosmotic shock. Two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Continuous lines were smoothed using R with a loess smoothing function. Shaded regions indicate 95% confidence intervals. d Normalized FRET ratio (DxAm/DxDm) time course of wild type BY4742 strain (blue), hog1∆::G418 mutant (gray), and pbs2∆::G418 mutant (black), live yeast cells expressing SED1, treated with 0.6 M sorbitol. The arrow indicates the addition of the treatment. Mean ± SEM. Two-way ANOVA. ***p < 2 ×10−16. Source data are provided as a Source Data file.
