Fig. 7: RNA-seq analysis of sympathetic ganglia from AdNT3-Tg and TRKC + /− mice after 1-day cold challenge.

a Heatmap of the expression of genes regulated in the opposite direction in sympathetic ganglia from thoracic T1 to T4 levels of AdNT3-Tg and TRKC + /− mice after 1-day cold challenge. T1–T4 sympathetic ganglia from six mice each group was pooled for RNA-seq analysis. b, c Real-time RT-PCR validation of the genes that are downregulated in sympathetic ganglia of AdNT3-Tg mice (b, n = 5 for WT (except for Tgm1 and Pgm1, where n = 4); n = 5 for AdNT3-Tg. * Indicates statistical significance between WT and AdNT3-Tg with unpaired two-tailed t test), but are upregulated in sympathetic ganglia of TRKC + /− mice (c, n = 5/group, * indicates statistical significance between WT and TRKC + /− with unpaired two-tailed t test) after 1-day cold exposure. d Measurement of neurite growth in sympathetic neurons treated with NT-3 (100 ng/ml) in the presence or absence of CXCL10 (100 ng/ml) (PBS n = 50, CXCL10 n = 43, NT-3 n = 47, CXCL10 + NT-3 n = 40. Statistical significance was analyzed by two-way ANOVA; with or without CXCL10 treatment, F (1, 210) = 95.47 P < 0.0001; with or without NT-3 treatment, F (1, 210) = 35.62 P < 0.0001. * Indicates statistical significance as shown in (d) with Turkey’s multiple comparisons test. Scale bar = 100 µm). Neurons from sympathetic ganglia at thoracic T1–T4 levels were isolated and used in this experiment. All data are expressed as mean ± SEM.