Fig. 7: Intranasal administration of wtNBD peptide inhibits α-syn spreading in PFF-seeded A53T mice. | Nature Communications

Fig. 7: Intranasal administration of wtNBD peptide inhibits α-syn spreading in PFF-seeded A53T mice.

From: Selective targeting of the TLR2/MyD88/NF-κB pathway reduces α-synuclein spreading in vitro and in vivo

Fig. 7

Propagation of α-syn in PFF-seeded mice brain was monitored in SN by immunostaining of pSyn129 and relative intensity measurement (a, b, p = 0.00001 for A53T + PBS vs A53T + PFF and p = 0.00001 for A53T + PFF vs A53T + PFF + wtNBD). Two sections from each brain were used for the staining and value obtained for each section is plotted in the graph. Spreading was also monitored by immunoblotting of total α-syn in Triton X-100 soluble (c, e, p = 0.0426 for A53T + PFF vs A53T + PFF + wtNBD) and insoluble fractions (d, f, p = 0.0067 for A53T + PBS vs A53T + PFF and p = 0.0078 for A53T + PFF vs A53T + PFF + wtNBD). The ratio of α-syn to actin is shown in the diagrams. Level of pSyn129 in the motor cortex was assessed by immunohistochemistry (g, h, p = 0.00003 for A53T + PBS vs A53T + PFF and p = 0.00017 for A53T + PFF vs A53T + PFF + wtNBD), where two sections from each brain were used for immunostaining and pSyn129-specific intensity was analyzed by Fiji. One-way ANOVA followed by Tukey’s multiple comparison tests was conducted for statistical analyses. **p < 0.01, ***p < 0.001 indicate significance compared to respective groups. Values are given as mean ± SEM (n = 4 animals per group).

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