Fig. 5: Peg acts directly on Wg protein.
From: Pegasus, a small extracellular peptide enhancing short-range diffusion of Wingless
a In wgNRT wing discs Wg (green) fails to diffuse away from the expressing cells, and Sens expression (red) is strongly reduced compared to wild type (Fig. 2c, quantifications in Fig. S2a-c, f). Note unaffected tsm. b wgNRT wings have fewer chemosensory bristles (*) (sometimes misplaced, arrowheads). c-f Neither the bristle phenotype, nor sens expression are affected by over-expression of PegGFP (c,d), or by Peg removal (e, f) (quantified in Fig. 2e). g-h. In-vivo FRAP imaging of WgGFP (activated by ptc-GAL4 UAS-Gal80ts, 24 h before dissection) before (pre), immediately after (t0, bleach), 30 seconds, and 5 min after photo-bleaching (performed between white lines), in a wild-type (g) or a peg- (h) background (see also Supplementary Movies 1 and 2). i FRAP quantification from panels g-h, showing average fluorescence intensity within the bleached region, over time, relative to the intensity before bleaching (pre). Acquisition was made every 30 s. Error bars represent SEM (n = 5 movies per genotype). Source data are provided as a Source Data file. Scale bars: a, c, e, g, h: 20 µm; b, d, f: 50 µm.
