Fig. 3: ADAR2 depletion affects homologous recombination.

A Effect of ADAR2 depletion in the DR-GFP reporter. A scheme of the reporter is shown on the top. Induction of a DSB using I-SceI meganuclease renders GFP-positive cells when the donor repeat (iGFP) is used in a gene conversion event. The efficiency of classical recombination (HR) was calculated as the percentage of GFP-positive cells in response to I-SceI expression upon downregulation of the indicated genes and normalized with the control. The average and standard deviation of at least three independent experiments are shown. B Same as A but using the Single Strand Annealing (SSA) reporter SA-GFP (top). In this case, the induction of a DSB located between two repeats in direct orientation will render GFP-positive cells only when intramolecular SSA takes place. C Same as A but using the non-homologous end-joining (NHEJ) reporter EJ5-GFP (Top). In this case, two I-SceI-induced DSBs could be repaired by conservative or mutagenic NHEJ granting the accumulation of functional GFP. In all panels, the average and SD of three independent experiments are shown and statistical significance was determined with a two-tailed paired Student’s t-test. Each individual replica is marked with a colored symbol. One, two, or three asterisks represent p < 0.05, p < 0.01, or p < 0.001, respectively. Actual p-values can be found in the Source data file. Only biological relevant comparisons are shown.