Fig. 3: Several viral neo-N-termini show sensitivity to specific protease inhibitors and a spike 637-proximal mutation alters viral entry in TMPRSS2-ve cells. | Nature Communications

Fig. 3: Several viral neo-N-termini show sensitivity to specific protease inhibitors and a spike 637-proximal mutation alters viral entry in TMPRSS2-ve cells.

From: Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential

Fig. 3

a Experimental design for N-terminomics of SARS-CoV-2 infection in the presence of protease inhibitors. b Abundance of viral neo-N-termini in infected cells ± inhibitors. Data normalised to total levels of the relevant viral protein. n = 3 biologically independent samples. Pseudovirus entry assay conducted in c HEK-Ace2 and d HEK-Ace2-TMPRSS2 cells. The infectivity of lentivectors (LV) pseudotyped with the different spike mutants was normalised to that of WT in the HEK-Ace2 cell line. n = 6 (RatG13 n = 5) biologically independent samples. e Western blotting of the pseudovirus stocks used in c and d confirms spike expression and incorporation into lentiviral particles. f Densitometry analysis of spike western blotting data, examining the ratio between uncleaved (S0) and cleaved (S1) portions of the spike protein present in purified pseudotyped lentivirus stocks (n ≥ 3 biological replicates). Boxplot minima/maxima represent the furthest non-outlier datapoints, centre the median, and bounds of box the interquartile range. Outliers are defined as datapoints >1.5 times the interquartile range from the bottom or top of the box. Unpaired Welch’s t-tests, which do not assume equal variance were used for statistical analyses.

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