Fig. 4: METTL3 supports NK cells accumulation and effector function in the TME.
From: METTL3-mediated m6A RNA methylation promotes the anti-tumour immunity of natural killer cells
a–d WT mice and cKO mice were harvested for flow-cytometric analyses 16 days after intravenous injection with 5 × 105 B16/F10 cells or PBS. a, c Representative plots showing expression of NK1.1 and CD3 in the liver (a) or lung (c) mononuclear cells from WT and cKO mice. b, d Percentages or absolute numbers of the liver (b) or lung (d) NK cells from WT mice (n = 6/group) and cKO mice (n = 6 for the control group; n = 4 for B16/F10 group). e Immunofluorescence staining of lungs with anti-NKp46 (red) and DAPI (blue) from WT or cKO mice 25 days after intravenous injection with 2 × 105 B16/F10 cells. Scale bar, 50 μm. The arrows indicate NK cells. f NKp46-positive cell numbers per field based on (e) (n = 6 for WT group; n = 5 for cKO group). g–l WT mice and cKO mice were harvested for flow-cytometric analyses 23 days after intravenous injection with 2 × 105 B16/F10 cells or PBS. Liver mononuclear cells were isolated and stimulated with PMA and ionomycin for 4 h for flow-cytometric analyses. Representative plots and percentages for the expression of CD107a (g, h), GzmB (i, j), and IFN-γ (k, l) expression among liver NK cells from WT (n = 5 for the control group; n = 6 for B16/F10 group) and cKO mice (n = 5 for the control group; n = 4 for B16/F10 group). Each symbol represents an individual mouse (b, d, h, j, l) or individual field of the microscope (f). Data are the mean ± SEM (ns, not significant; unpaired two-tailed t test (f) or one-way ANOVA (b, d, h, j, l)). Source data are provided as a Source Data file. Data represent at least two independent experiments (a–l).
