Fig. 7: PAX7 loss rescues deleterious effects of Pten loss in ASP^cKO tumors.

a Breeding strategy to generate ASP^cKOP7^cKO tumor mice. b Kaplan–Meier tumor-free survival curve of ASP^cKOP7^cKO (n = 32) in comparison to ASP^WT and ASP^cKO mice (from Fig. 1b). Mantel–Cox log rank, P < 0.0001. c Tumor mass of ASP^cKOP7^cKO (n = 10), ASP^WT (n = 11) and ASP^cKO (n = 12) tumors at P12. ASP^WT and ASP^cKO data are the same from Fig. 1c. (ASP^WT-ASP^cKO P = 0.0022, ASP^cKO-ASP^cKOP7^cKO P = 0.0056). d Representative histology of ASP^cKOP7^cKO (n = 14), ASP^cHetP7^cKO (n = 4), and ASP^cKOP7^cHet (n = 4) tumors by H&E staining and IHC for DESMIN, MYOD1, and MYOG. Scale bar, 25 μm. e Quantitation of percent Ki67-positive nuclei in ASP^WT (n = 9), ASP^cKO tumors (n = 7), and ASP^cKOP7^cKO (n = 5) tumors (ten random fields of view per genotype). ASP^WT and ASP^cKO from Fig. 1f. (ASP^WT-ASP^cKO P = 0.0029, ASP^WT-ASP^cKOP7^cKO P < 0.0001, ASP^cKO-ASP^cKOP7^cKO P < 0.0001). f Immunoblots of phosphorylated AKT^Ser473, AKT, phosphorylated S6^Ser235/236, S6, and GAPDH, n = 3. All P values in pairwise comparisons were determined by Student’s t test (unpaired, two-tailed); NS   not significant, **P < 0.01, ****P < 0.0001. Data represented as mean + SEM. Source data are provided as a Source Data file.