Fig. 1: RFS-1/RIP-1 stimulates formation of RAD-51-ssDNA filaments in the presence of ATP or ADP. | Nature Communications

Fig. 1: RFS-1/RIP-1 stimulates formation of RAD-51-ssDNA filaments in the presence of ATP or ADP.

From: Nucleotide proofreading functions by nematode RAD51 paralogs facilitate optimal RAD51 filament function

Fig. 1

Average normalized Cy3-43mer fluorescence profiles plotted as a function of time. The arrow indicates the components of the two syringes rapidly mixed at the 0 s time point in a stopped flow instrument. In all experiments RAD-51 (250 nM) alone or in the presence of RFS-1/RIP-1 (10 and 50 nM) was mixed with Cy3-43mer ssDNA (15 nM). a and e 5′-Cy3, (b and f) Int(21)-Cy3 or (c and g) 3′-Cy3 labelled substrates were used (a: n = 5, b: n = 4–5, c: n = 4–5, e: n = 4, f: n = 4–8, g: n = 5–6). Schematics of these different Cy3 label positions are shown inset. d and h Graphs of RFS-1/RIP-1 concentration-dependence of average Δ Cy3 fluorescence for the data presented in (a–c) and (e–g) respectively (mean; errors: s.d.). Experiments in (a–d) were performed in the presence of ATP, whereas (e–h) were performed with ADP. Source data are provided as a Source Data file.

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