Fig. 6: Interactions of the DNA-binding β-hairpins with the 3′-ssDNA.
From: Unwinding of a DNA replication fork by a hexameric viral helicase
a Top left, a cross-section of E1RF viewed from the top showing the fitted atomic model of the six DNA-binding β-hairpins in the EM map (grey mesh). The bottom left panel displays a side view of the same area revealing the staircase organisation of the β-hairpins. The right panels show the same slices of the atomic models with amino acid side chains to show interactions with the 3′ ssDNA. b Overlay of the DNA-binding β-hairpins from the fitted atomic model of the E1RF cryo-EM map (in colours corresponding to the E1 subunits) and the X-ray structure of E1HD/ssDNA/ADP14 (in grey, PDB 2GXA). On the right, a cartoon illustrates the step-wise organisation of the individual DNA-binding β-hairpins in each structure. His507s are shown and their relative heights within the central channel of the AAA+ domain.
