Fig. 1: TAG content and LD abundance are dramatically decreased in M1-7 mutant. | Nature Communications

Fig. 1: TAG content and LD abundance are dramatically decreased in M1-7 mutant.

From: Sterols are required for the coordinated assembly of lipid droplets in developing seeds

Fig. 1

a Phenotypes of 4-week-old M1-7 and Col/OLE1. Bar = 2 cm. b Representative images of LDs (OLE1-GFP, green) in rosette leaves of different ages of 4-week-old plants (young leaves: the upper growing leaves; mature leaves: the newest expanded leaves; old leaves: the oldest expanded green leaves). The experiment was repeated 5 times with similar results. Bar = 50 μm. c Thin-layer chromatogram of neutral lipids in leaves of M1-7 and Col/OLE1. Lipids were visualized with 5% sulfuric acid by charring. d Relative TAG contents in leaf of M1-7 and Col/OLE1 quantified by Image J software. Data are mean ± SD. of four biological replicates. Different letters indicate significant differences at P < 0.05, as determined by one-way ANOVA with Tukey’s multiple comparisons test.

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