Fig. 1: NHSL1 localises to the very edge of lamellipodia. | Nature Communications

Fig. 1: NHSL1 localises to the very edge of lamellipodia.

From: Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration

Fig. 1

a Northern blot analysis of NHSL1 expression in different murine tissues. Arrows show a large 7.5 kb isoform that is ubiquitously expressed and various shorter isoforms with varying tissue specific distribution. b–d Western blots detecting NHSL1 protein in the indicated cell lines using the b, c polyclonal (4457) or d monoclonal antibody (C286F5E1). For full blots see Supplementary Fig. 1a–c. e Scheme of NHSL1 protein (grey rectangle) containing three putative SH3 binding sites (indicated black boxes). GST-fusion proteins 1–8 and EGFP-fusion proteins 1–4 of NHSL1 are indicated (white rectangles). Immunogen for the NHSL1 polyclonal antibody is indicated. f, g N-terminal EGFP-tagged NHSL1 (f) and C-terminal EGFP-tagged NHSL1 (g) were expressed in B16-F1 cells and, after plating on laminin, localisation of both EGFP-tagged NHSL1 constructs were imaged live. Scale bar: 20 μm. Inset represents a magnified view of the white box. Scale bar in the inset: 1.25 μm. Representative blots or images shown from a one and b–d, f, g three independent biological experiments. See also related Supplementary Movie 1.

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