Fig. 4: NHSL1 co-localises with Abi and the Scar/WAVE complex. | Nature Communications

Fig. 4: NHSL1 co-localises with Abi and the Scar/WAVE complex.

From: Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration

Fig. 4

a Still images from live-cell imaging showing NHSL1-EGFP co-expressed with mScarlet-I-tagged Abi1 and b Nap1 in B16-F1 cells. Representative images shown from three independent experiments. Scale bar represents 20 µm. Inset is a magnified view of the white box. Scale bar in inset a applies to both insets a, b: 1.25 μm. See also related Supplementary Movie 6. c–e Endogenous NHSL1 co-localises with Abi1 (c NHSL1 pAb) Scar/WAVE1 (d NHSL1 pAb), and Scar/WAVE2 (e NHSL1 mAb) at the very edge of lamellipodia in B16-F1 mouse melanoma cells. Scale bar in c applies also to d, e: 20 µm. Inset represents a magnified view of the white box. Scale bar in the inset in c applies also to inset for d, e: 20 μm. Representative images shown from three independent experiments. See Supplementary Fig. 8 for line scans of co-localisations in c and e.

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