Fig. 2: daf-18(yh1) improves health parameters in daf-2(−) mutants.

a–d Percent changes in developmental time (n ≥ 177 for each condition, from two independent trials) (a), total brood size (n ≥ 37 for each condition, from five independent trials) (b), swimming rate at day 0 (n = 90 for each condition, body bends per minute in liquid measured from nine independent trials) (c), and feeding rate at day 0 (n = 50 for each condition, pharyngeal pumping per minute measured from five independent trials) (d) of wild-type (WT), daf-2(e1370) [daf-2(−)]; daf-18(+), daf-2(−); daf-18(yh1), and daf-2(−); daf-18(nr2037) [daf-18(−)] animals. e–i Indicated health span of WT, daf-2(−), daf-2(−); daf-18(yh1), and daf-2(−); daf-18(−) animals. Shown are swimming span (n = 10 for each condition, from one trial) (e), motility span (n = 20 for each condition, from two independent trials) (f–h), and feeding span (n = 10 for each condition, from one trial) (i). Class A worms (green) are healthy and mobile, class B worms (yellow) display intermediate phenotypes, mobile but irregular movement, and class C worms (red) are immobile, in (f–h)⁷¹. See Supplementary Fig. 2f, g for the effects of daf-18(yh1) and daf-18(−) on the swimming span and feeding span in the WT background. Error bars indicate the standard error of the mean (s.e.m., *p < 0.05, **p < 0.01, ***p < 0.001, n.s.: not significant, two-tailed Student’s t test relative to WT unless otherwise noted). See Supplementary Dataset 4 for additional repeats and statistical analysis for the health span assay data shown in this figure. See also Source Data for data points used for the derivation of data.