Fig. 5: Control over PDAC cell phenotypes in PA hydrogels.
From: Bioengineered 3D models of human pancreatic cancer recapitulate in vivo tumour biology
a Gene expression of PDAC cells (12560) as a function of PA concentration relative to 2D controls (mean ± SD; n = 3 biological replicates). p values were calculated by two-way ANOVA with Bonferroni correction. b Percentage of vimentin-positive PDAC colonies (12560) as a function of PA concentration as quantified from immunofluorescence micrographs. Box plots indicate range, interquartile range and median (n = 8 biological replicates). p values were calculated by one-way ANOVA with Bonferroni correction. c Log2 normalised expression of PDAC cells (12560) grown in PA hydrogels assembled in different gelling conditions relative to the 2D baseline (mean ± SD; n = 3 biological replicates). p values were calculated by two-way ANOVA with Bonferroni correction. d Log2 normalised gene expression of PDAC cells (12560) cultured in PA hydrogels in sphere medium (SM) and PSC-conditioned medium (PCM) relative to the 2D SM baseline (mean ± SD; n = 3 biological replicates). p values were calculated by two-way ANOVA with Bonferroni correction. e Scratch closure of PDAC cells (12560) cells in the presence of hyaluronan. Box plots indicate range, interquartile range and median (n = 12 biological replicates in three experiments). f Log2 normalised invasion of PDAC cells (12560) across collagen-coated transwells as a function of the concentration of low (10 kDa) and high (1 MDa) molecular mass hyaluronan. Box plots indicate range, interquartile range and median (n = 42 biological replicates in 6 experiments). p values were calculated by one-way ANOVA with Bonferroni correction. g Number of spheres formed by PDAC cells (12560) as a function of the size and concentration of hyaluronan in the medium (mean ± SD; n = 6 biological replicates in three experiments). p values were calculated by two-way ANOVA with Bonferroni correction. h Brightfield images of PDAC cell colonies (12560) formed on low-attachment plates coated with collagen, with and without 10 kDa/1 MDa hyaluronan in the medium. Scale bars: 500 µm.
