Fig. 2: Specific proteins exhibit unique modes of EV release.

a Comparison of EV protein content identified with LC-MS from ciliated (serum-starved) and less ciliated (serum-fed) KM cells showed less proteins released from serum-fed cells. b, c Proteins identified via liquid chromatography-mass spectrometry analysis in four out of six (smEV and mixed EV) or three out of four (lgEV) pellets isolated from ciliated control KM supernatant. Each point represents one protein. Its location on the x axis depicts its relative abundance. b Proteins listed on the ExoCarta Top 100 protein list are marked in blue (exocarta.org). ExoCarta proteins were predominantly found in the middle cluster, therefore equally abundant in small vs. large EVs. c Proteins localising to the left cluster were considered enriched in lgEVs and absent from smEVs. Significantly enriched lgEVs (relative abundance ≤ −10 and a significance −log ≥2) are labelled red. Significantly enriched lgEVs that were also identified in separate lgEV preparations are labelled purple. d Absolute numbers of different protein populations depicted in (c, d). e In all, 93 out of the 100 ExoCarta proteins were detected. f Graphical representation of smEV protein abundance. g Absolute numbers of Wnt signalling proteins identified in different populations as determined by Uniprot or GetGo (uniport.org; getgo.russelllab.org). b–f Source data are provided as Source and Supplementary data file.