Fig. 2: Grp78 is a sugar-induced inhibitory partner for AdipoR.

a Grp78 immunodetection in the hemolymph of low (LSD) and high (HSD) sugar diet-fed larvae. Cv-d is a control for hemolymph proteins. (the experiment was repeated three times independently with similar results). b grp78 RNA levels in the whole larva in LSD (grey) versus HSD (red). qRT-PCR profiles, fold changes (FC) normalized to rp49. sugarbabe serves as a control for sugar induction. Statistical significance was tested using ordinary two-way ANOVA with Sidak’s multiple comparisons test. n = 3 biologically independent replicates per experiment (two independent experiments). c Co-immunoprecipitations (co-IP) of AdipoR-Myc, Grp78-HA, and hAdipoQ-Flag expressed in S2 cells. Inputs (lanes 1–4; 15–18). Myc (M), HA (H), and Flag (F) IPs are used to visualize AdipoR-Myc (5–14) or Grp78-HA (19–28). (the experiment was repeated three times independently with similar results). d Brain from Apn > CaLexA larvae fed in LSD and incubated with the hemolymph from lpp > control larvae (LSD- n = 25, orange or HSD-fed n = 37, red points) or lpp > grp78-Ri larvae (−40%; HSD-fed n = 29, red squares). d′ Quantification of GFP fluorescence in APN. n = independent averaged GFP intensity measurements in APN. Statistical significance was tested using a two-sided Mann–Whitney test. Data were presented as mean values ± SEM. P values are indicated in all panels. a.u. arbitrary units. Source data are provided as a Source Data file.