Fig. 3: Central AdipoR function controls JH signaling.
From: Brain adiponectin signaling controls peripheral insulin response in Drosophila
a Anti-AdipoR and anti-GFP immunostaining of Apn > mCD8-GFP larval brains. Brain and ring gland are stained with DAPI, the corpora allata are circled. (n = 18 in five independent experiments). b, c jhi-26 and kr-h1 expression on whole larvae Apn > AdipoR-Ri (red) and Apn > (black). qRT-PCR profiles, fold changes (FC) normalized to rp49. Statistical significance was tested using ordinary two-way ANOVA with Sidak’s multiple comparisons test. n = 2–3 biologically independent replicates per time point (three independent experiments). d Measure of JH analog (pyroproxyfen)-feeding wing disc size. (control n = 22, black; control w/Jha n = 21, red, in three independent experiments). Statistical significance was tested using ordinary two-way ANOVA with Sidak’s multiple comparisons test. e Measure of adult weight in JH analog feeding (control n = 7, black; control w/Jha n = 8, red). Statistical significance was tested using a two-sided unpaired t-test with Welch’s correction. f, g Rescue of Apn > AdipoR-Ri female lethality (f) and adult weight (g) by met27, gce25k heterozygous mutations (met27, gce25k/FM7; Apn > AdipoR-Ri in red compared to Apn > AdipoR-Ri in light gray). n = 4 independent experiments (in f), n = 3 groups of 15 females of each genotype (in g). Statistical significance was tested using two-sided unpaired t-test with Welch’s correction. Data were presented as mean values ± SEM. P values are indicated in all panels. a.u. arbitrary units. Source data are provided as a Source Data file.
