Fig. 5: Spatial immunophenotypes are characterized by distinct T cell evasive mechanisms. | Nature Communications

Fig. 5: Spatial immunophenotypes are characterized by distinct T cell evasive mechanisms.

From: Spatial immunophenotypes predict response to anti-PD1 treatment and capture distinct paths of T cell evasion in triple negative breast cancer

Fig. 5

a Representative images of cells and molecules related to spatial phenotypes (spatial phenotype panel) at the tumor border and center (CD11b (orange), CD163 (green), CD8 (red), CK (cyan), CLEC9A (yellow), or S100A7 (magenta); scalebar corresponds to 50 μm). b Circle plots show mean and SD of cell densities at border and center regions per mm2 for CD11b (orange), CD163 (green), CD8 (purple), CK (cyan), CLEC9A (yellow), or S100A7 (magenta); Collagen-10 (pink) is displayed as positive tissue area in μm2/100 for visualization purpose (n = 68 TNBC comprising n = 20 excluded, n = 22 ignored, and n = 26 inflamed). c Histograms show mean distances in μm between CD8+ T cells and CD11b (orange), CD163 (green), CD8 (purple), CK (cyan), CLEC9A (yellow), or S100A7 (magenta) (x-axis) versus cell densities (y-axis) (n = 68 TNBC of which n = 20 excluded, n = 22 ignored, and n = 26 inflamed). d Boxplots show median with 25th–75th percentile and range (outliers are displayed as dots) of numbers of high endothelial venules (HEV, identified via MECA-79 staining, black arrow) and MHC-II expression of tumor cells for excluded (cyan), ignored (green), and inflamed (purple) TNBC (no distinction between border and center, pink arrow: tumor cells; yellow arrow: adjacent normal breast lobules; green arrow: immune cells, scalebar corresponds to 100 μm, n = 20 TNBC, of which n = 6 excluded, n = 4 ignored, and n = 10 inflamed). e Boxplots show median with 25th–75th percentile and range (outliers are displayed as dots) of neutrophil densities (CD66b+) at border and center for excluded (cyan), ignored (green), and inflamed (purple) TNBC and the representative image is shown, scalebar corresponds to 100 μm, (n = 32 TNBC, of which =11 excluded, n = 10 ignored, and n = 11 inflamed). f Boxplots show median with 25th–75th percentile and range (outliers are displayed as dots) of numbers of different T cell markers stained on consecutive slides, and representative images (CD8 (purple), CD4 (green), 41BB (cyan), and ICOS (yellow); scalebar corresponds to 100 μm, n = 20 TNBC, of which n = 6 excluded, n = 4 ignored, and n = 10 inflamed). Significant differences are: ***p < 0.001; **p < 0.01; *p < 0.05, NS, p > 0.05 (Kruskal–Wallis, one-sided). Source data are provided within source data file.

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