Fig. 1: Schematic of the membrane-less protocell array platform for simultaneous detection of multiple analytes.
From: Protocell arrays for simultaneous detection of diverse analytes
The use of a spatially patterned array of phase-separated protocell sensors enables each microwell to provide measurements for multiple analytes using a single fluorescent or colorimetric reporter. a An overhead view of a microwell with four micro-basins. White area surrounding the micro-basins (yellow-shaded regions) represents the bulk phase formed by a PEG solution containing the analytes to be detected. Each micro-basin has a protocell containing a CFE reaction (represented by the microcentrifuge tube) with a different sensor plasmid or genetic circuit. b Side view of a microwell with micro-basins. Analytes in the bulk phase (blue diamonds) enter protocells to activate their cognate sensor (blue DNA construct), producing detectable reporter signals. Multiple analytes can be measured in parallel by tracking which protocell produces reporter signals (green color on the right).
