Fig. 6: UBA5 linker affecting UFC1 active site Cys.
a–b Chemical shift perturbations (CSPs) of 0.2 mM 15N-labeled UFC1 upon addition of equimolar concentration of UBA5347–404 (a) or five molar equivalents of UBA5347-392 (b). Bars correspond to UFC1 active site C116 and F121 are colored in red. Select regions of 1H–15N HSQC spectra showing the spectrum of 0.2 mM UFC1 alone (black) and in the presence of an equimolar concentration of UBA5347-404 (red; a, bottom row) or five-fold excess of UBA5347-392 (purple; b, bottom row) c Structure of UFC1 (pdb 2Z6P) with the residues displaying significant CSPs (>0.04 ppm) upon interaction with UBA5347-404 (upper panel) or UBA5347-392 (>0.008 ppm; lower panel) colored in light red. For upper panel residues with CSPs value greater than 0.08 ppm are highlighted in red. Yellow and black arrows indicate the positions of UFC1 active site C116, or UFC1 F121, respectively.
