Fig. 3: Monomeric POI encapsulation using tES–F116H.
From: A general approach to protein folding using thermostable exoshells
a tES–F116H can encapsulate three rFasxiator molecules (36 kDa, total, loading ratio of 60:15) (AUC peaks are labeled with the number of encapsulated POI with colors corresponding to the molar ratios of titrated POI during the folding protocol). b tES–F116H charge is a critical determinant of encapsulation efficiency. Positively charged rFasxiator exhibits no encapsulation in tES–F116H(+). c Two rLuc molecules (72 kDa, total, loading ratio of 60:10) can be accommodated in tES–F116H. d Negatively charged rLuc shows optimal encapsulation in tES–F116H(+). e tES accomodates one HSA (66 kDa, total, loading ratio of 60:5) molecule. f Negatively charged HSA shows optimal encapsulation in tES–F116H(+).
