Fig. 4: N-terminus of Tim23 is localized in proximity to the TOM complex.
From: Mapping protein interactions in the active TOM-TIM23 supercomplex
a Mapping of cross-links obtained between Jac1sfGFP and various supercomplex subunits, indicating its relative position within the supercomplex. b Structural model representation of Tim23 relative to the TOM complex, indicating cross-links (red dashed line) between Tim23 (K25, gray), Tom40 (K148, green), and Tom5 (K50, yellow) in the intermembrane space (IMS). Red helix represents the region of Jac1sfGFP predicted to be localized in the IMS based on (a). The model utilizes structural information from available TOM complex: 6JNF and Tim23: 7CLV structures. The color code indicates individual subunits. c Close-up view of the Tim23-Tom40 and Tim23-Tom5 cross-links. d Illustration showing Tim21-Tom22 cross-links using the structure of the IMS domains of Tim21 (cyan, PDB id: 2CIU). e Interaction between Tim50-Tim23 and Tim50-Tom22 based on cross-links. Tim50 (mustard) is indicated as a combination of the known structure of Tim50CORE (PDB id: 3QLE) and an ab initio predicted model for the C-terminal domain. OMM: outer mitochondrial membrane, IMS: intermembrane space, IMM: inner mitochondrial membrane.
