Fig. 4: The NEPC subtypes are distinct clones.
From: Subtype heterogeneity and epigenetic convergence in neuroendocrine prostate cancer
a Genome-wide CNV profiles inferred from the scATAC-seq clusters in FLM3 and FLM5. Black dots are values in 1 MB regions and the red line is the result of running a segmentation algorithm on the data (see “Methods”). Arrows point to differences seen in CNVs across the clusters. b Sample pairwise Pearson’s correlation of the CNV profiles. c Summary heatmap of the scATAC-seq-inferred CNV alterations across all of the patient samples (blue represents losses and red represents gains). d Heatmap of the single-cell CNV analysis of FLM3 where each column is a 2 MB bin tiled across the genome and the rows are individual cells that have been clustered with K-means. Arrows point to CNV differences observed here and in the cluster level analysis. e tSNE plot of FLM3 scATAC-seq data colored by the cluster each cell was partitioned into by the inferred CNV alterations. Those three clusters clearly correspond to NEUROD1 (blue), ASCL1 (green), and normal cells (gray). Source data are provided as a Source Data file.
