Fig. 2: LECs display anatomically segregated and temporally-dynamic APC activity.
a Histogram and quantification of in vivo DQ-ovalbumin (DQ-OVA) uptake and processing abilities of LECs from naïve and Sp19F-infected mice 48hours postinfection (hpi), two-tailed Mann–Whitney test. b Histogram and quantification for LEC MHC-II from naïve and Sp19F-infected mice 48hpi, two-tailed Mann–Whitney test. c Genetic construct and experimental timeline of MHC-IIΔEpi mice. Quantification of OT-II CD4+ T cell activation by MHC-IIfl/fl and MHC-IIΔEpi LECs sorted from Sp19F-infected mice 48 h postinfection (hpi), one-tailed Unpaired t test. Sorting strategy in Supplementary Figure. 2c. Note, different scales for Y-axes in Fig. 2a–c highlight that all LECs are capable of antigen presentation albeit in different amplitudes based on their anatomical location. d Schematic of experimental timeline. e opt-SNE plot depicting 6 LEC metaclusters identified from ~2 million LECs isolated from n = 84 mice across nine timepoints each with two independent experiments. f Heat map depiction for expression of APC-related molecules mapped onto opt-SNE projection. Temporal quantification for g MHC-II, h CD40, i. CD86, j ICAM1, k PD-L1, and l PD-L2 across distinct LECs. Two- Way ANOVA with Fisher’s LSD test statistics for Fig. 2g–l are provided in Supplementary Table 1-6. All data have n ≥ 4 mice, two independent experiments. All data are presented as mean ± SEM.
