Fig. 5: Functional and transcriptional subtype heterogeneity in liver metastases. | Nature Communications

Fig. 5: Functional and transcriptional subtype heterogeneity in liver metastases.

From: Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy

Fig. 5

a For one patient, organoid lines were derived from the primary tumor (P) as well as two liver metastases (M1 and M2). Shown is a UMAP representation of transcriptomes integrated by reciprocal PCA, with shared and organoid line-specific cell clusters identified by unsupervised clustering. Cells are colored by sample origin (left) and cluster membership (right). b Expression of genes involved in cell cycle (MKI67), secretion and digestion (FABP1, PRSS1, and PGC), and immune regulation (CD70, TMEM176B) across cells from P, M1, and M2 organoids. c Expression of the top 10 enriched genes for each cluster. d Moffitt subtype scores from bulk RNA-seq data (left) and single-cell RNA-seq data (right) for P, M1, and M2 organoids. Blue indicates classical subtype scores, red basal-like (“Methods”). Cells are grouped by cluster identity according to (a) in the violin plots. e Projection of M1 and M2 transcriptomes onto the primary PDAC organoid data (see Fig. 4f and “Methods”) shows the distribution of cells across cycling and differentiating clusters.

Back to article page