Fig. 6: Classical subtype gene expression correlates with organoid drug responses. | Nature Communications

Fig. 6: Classical subtype gene expression correlates with organoid drug responses.

From: Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy

Fig. 6

a Overview of the experimental drug screen protocol. PDAC organoids were treated with a range of drug concentrations over 72 h, and then subjected to 3D confocal imaging. By measuring the intensity of Hoechst (H) and propidium iodide (PI) signals, scores were computed to compare drug-induced cell death and proliferation inhibition. Scale bars, 200 µm. b Correlation between median subtype scores calculated from scRNA-seq data and drug-induced proliferation inhibition, as measured by the area under curve (AUCpi), for 24 PDAC organoid lines. A higher score on the x-axis represents a more ‘classical’ transcriptional phenotype. c Overview of variation in transcriptomic PDAC subtype (top), median cell cycle score (middle), and proliferation inhibition in response to 5-FU treatment (bottom), shown as z-scores scaled across all samples.

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