Fig. 3: Micronuclei in embryonic cells undergo defective DNA replication.
From: Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing
a Scheme for the experiment. hCG human Chorionic Gonadotropin. b Left, representative single z-focal plane image of blastomeres, quantified in the right panel, by brightfield confocal microscopy and corresponding fluorescence DAPI and EdU-labeling. The red arrow points to a micronucleus (MN) detected by DAPI staining that fails to incorporate EdU, despite active replication in the primary nucleus (PN). Scale-bar: 10 μm. Right, quantification of the ratio of density of EdU signal in micronuclei relative to primary nuclei. If micronuclei replicate normally this ratio will be one (dotted red line). Strongly reduced DNA replication was evident in most micronuclei. Error bars: mean ± SEM (n = 1 experiment with 36 micronuclei scored). Source data are provided as a Source Data file.
