Fig. 6: Residues involved in the substrate binding pocket. | Nature Communications

Fig. 6: Residues involved in the substrate binding pocket.

From: Cryo-EM structures of the ABCA4 importer reveal mechanisms underlying substrate binding and Stargardt disease

Fig. 6

a N-Ret-PE is wedged between transmembrane domains TMD1 and TMD2 and B-loop. The residues that interact with the substrate are indicated as purple sticks. R653 (TM2) and R587 (ECD1) form ionic interactions with the phosphate group of N-Ret-PE. The interactions include several aromatic residues in B-loop (W339, Y340, F348). The β-ionone group interacts with Y345 (B-loop), L1674 (TM8), S1677 (TM8), L1812 (TM11) and L1815 (TM11). b Orthogonal view of the binding pocket showing the residues involved in the binding to phosphate and residues belonging to TMD2. c Residues in the B-loop as viewed from the exocytoplasmic domain.

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