Fig. 5: BRON regulates cell division in response to BR. | Nature Communications

Fig. 5: BRON regulates cell division in response to BR.

From: Integrated omics networks reveal the temporal signaling events of brassinosteroid response in Arabidopsis

Fig. 5

a RT-qPCR of BRON (left, blue) and VDOF2 (right, orange) in mutant lines. Reported values are 2−ΔΔCΤ. Centerline represents the median; box bounds represent 25th and 75th percentiles; whiskers represent minimum and maximum. Colored dots represent the average of two technical replicates for each biological replicate (n = 3 except for tmk1-1, where n = 3 were assayed but BRON was detected in only n = 2). ND: not detected. * denotes p < 0.05, *** p < 0.0001 using two-tailed z test compared with mean and standard deviation of WT. Multiple testing correction was not performed. b Proposed model for the role of BRON in BR response. The BR-responsive kinases MAP4Kα1, BAK1, MPK6, and TMK1 regulate BRON. In addition, BRON and VDOF2 regulate each other in a negative feedback loop. The coordination of these upstream regulators results in the repression of BRON after BL treatment. This repression of BRON lifts its repression on the cyclins CYCD3;1 and CYCP4;1, leading to BR-induced cell division.

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