Fig. 3: Electrical recording during optogenetic inhibition of eNpHR3.0-expressing neurons. | Nature Communications

Fig. 3: Electrical recording during optogenetic inhibition of eNpHR3.0-expressing neurons.

From: Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Fig. 3

a AP traces were recorded in the M2/VO cortex of mouse M01 by an AAV9-hSyn::eNpHR3.0-delivery optrode at 2 weeks after implantation (left), from which 27 neurons were isolated (right). The yellow line indicates the duration of 10-s yellow light stimulation. Scale bar, 800 μV (vertical, left), 2 s (horizontal, left), 100 μV (vertical, right), and 1 ms (horizontal, right). b Spike rasters of 27 neurons in M01 during yellow light stimulation. Scale bar, 2 s. c AP traces were recorded by an example channel (Ch20) in response to 10-s yellow light stimulation at 2–20 mW/mm2. Scale bar, 100 μV (vertical) and 2 s (horizontal). d Averaged firing rates of neurons recorded by Ch20 under 2–20 mW/mm2 stimulation (from up to down). The firing rates were binned at 2 Hz. e, f Percentage of neurons with a decrease or an increase in firing rates during 20-mW/mm2 stimulation (n = 2 mice in each group). Neurons were transduced with eNpHR3.0 via VVD-optrodes in (e) and solution injections in (f), respectively. The right panels are schematics showing the neuronal transduction characteristics of AAV-delivery probes and conventional solution injections.

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