Fig. 1: EThcD analysis of in vitro ADP-ribosylated PARPs.
From: The regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome
a Overview of the experimental design. Recombinant PARP1, PARP10, or PARP14, were modified in vitro. For PARP1, the reaction was performed ±H1.0 and ±HPF1. Samples were trypsinized, and afterward mock-treated or treated with PARG, prior to analysis of the peptides by ETD-based MS. b Total abundance distribution of in vitro ADPr-modified amino acid residues. Abundance values ± PARG were summed. c Average abundance distribution of in vitro auto-modified residues on PARP1. n = 3 technical replicates. “n.d.” not detected, data points not shown in case of n.d. d Fully annotated EThcD MS/MS spectrum, demonstrating confident localization of ADP-ribosylation to Glutamate-491 on PARP1. Light blue; b-ions, dark blue; c-ions, red; y-ions, orange; z-ions, gray; unassigned. Source data are provided as a Source Data file.
