Fig. 1: The ER-phagy targeting complex involves RTN3 engagement of PGRMC1.
From: PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones
a Schematic of the topology of RTN3. b Total number of peptides corresponding to PGRMC1 identified by mass spectrometry on materials obtained from FLAG IP of HEK 293 T cells expressing 3xFLAG-GFP-Sec61B, 3xFLAG-GFP-RTN3C, or 3xFLAG-GFP-RTN4A. c HEK 293 T cells were treated with the cross-linker DSP and subject to lysis. IP was performed using either anti-RTN3 or IgG antibody, and the precipitated material was subjected to SDS-PAGE followed by immunoblotting with the indicated antibodies. N = 3 independent experiments. d HEK 293 T cells expressing HA-PGRMC1 were co-transfected with FLAG-ERLIN1, FLAG-RTN3A, or FLAG-RTN3C. Cells were lysed and the resulting lysate incubated with a FLAG antibody. The immunoprecipitated material was eluted with excess 3xFLAG, subjected to SDS-PAGE followed by immunoblotting with the indicated antibodies. N = 3 independent experiments. Source data are provided as a Source Data file.
