Fig. 3: 3′UTR ribosomes guide piRNA 5′-end formation.
From: Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs
a Normalized reads of anti-HA-immunoprecipitated RPFs and piRNAs mapping to four representative uppl genes in adult testes. b Residual plot of partial correlation between piRNA and RPF abundance conditional on RNA-seq abundance at the uppl ORFs (left) and 3′UTRs (right) in adult testes. Linear regression was performed between piRNA and RNA-seq abundance and between RPF and RNA-seq abundance separately, and residuals were plotted with smooth parameter “method = lm”. Data are presented as mean values ± standard deviation shown as error bands. c Distance spectrum of 5′-ends of RPFs (26–32 nts) from ORFs (left) and from 3′UTRs (right) that overlap piRNAs in adult testes (sample size n = 3 independent biological replicates). Data are mean ± standard deviation.
