Fig. 2: Ketamine reduces compulsive grooming in SAPAP3 KO mice.

a Schematic of experiment. b KO mice have higher baseline grooming frequency compared to WT controls. Ketamine reduces grooming frequency in KO animals 1-h and 1-day post-injection (two-way RM ANOVA: interaction P = 0.0002, time P = 0.0250, experimental group P < 0.0001, subject P < 0.001; significance derived from Tukey’s multiple comparisons are marked with asterisk(s); see Supplementary Fig. 4 for exact P values). c KO mice given ketamine have reduced grooming frequency compared to KO mice given saline (two-way RM ANOVA: interaction P = 0.0015, time P > 0.05, experimental group P = 0.0115, subject P < 0.0001; Sidak’s multiple comparisons: 1-h timepoint P = 0.0002, 1-day timepoint P = 0.0138). d WT mice show no change in grooming frequency between treatment groups (two-way RM ANOVA: interaction P = 0.7892, time P < 0.0001, experimental group P = 0.7173, subject P < 0.0001). e Ketamine attenuates grooming duration in KO animals 1-h post-injection. Saline injection increases KO grooming compared to WT controls 1 h, 1 day, and 3 days post-injection (two-way RM ANOVA: interaction P = 0.0081, time P = 0.5614, experimental group P < 0.0001, subject P < 0.0001; significance derived from Tukey’s multiple comparisons are marked with asterisk(s); see Supplementary Fig. 4 for exact P values). f KO-ketamine mice have decreased grooming duration compared to the KO saline group (two-way RM ANOVA: interaction P = 0.0208, time P = 0.7935, experimental group P = 0.0040, subject P = 0.0864; Sidak’s multiple comparisons: 1-h timepoint P = 0.0026, 1-day timepoint P = 0.0120). g WT mice displayed no difference in grooming between treatment groups (two-way RM ANOVA: interaction P = 0.4333, time P = 0.0069, experimental group P = 0.7587, subject P < 0.0001). Bar and line graphs show data means +/− SEM. WT saline = 14, WT ketamine = 13, KO saline = 11, KO ketamine = 12. Source data are provided as a Source data file. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.