Fig. 5: Optogenetic inhibition of dmPFC-DMS projections increases grooming in WT mice. | Nature Communications

Fig. 5: Optogenetic inhibition of dmPFC-DMS projections increases grooming in WT mice.

From: Ketamine increases activity of a fronto-striatal projection that regulates compulsive behavior in SAPAP3 knockout mice

Fig. 5

a WT mice are bilaterally injected with CaMKIIα-eNpHR3.0 virus in the dmPFC and implanted bilaterally with optical fibers in the DMS. b Raster-like plot of grooming behavior for individual animals across experimental timeline (top: eNpHR3.0; bottom: eYFP control). Each row represents one animal. Dark green dashes indicate grooming timestamps and light green shading indicates when laser was on (8 mice per group). c eNpHR3.0 mice began grooming significantly sooner than eYFP mice after laser onset (unpaired two-tailed t-test P = 0.0150). d eNpHR3.0 mice (N = 8) show increased grooming during laser on epochs that is significantly higher than eYFP controls (N = 8) (two-way RM ANOVA: interaction P = 0.0395, laser P = 0.0841, virus group P = 0.0010, subject P = 0.1264; Sidak’s multiple comparisons: eNpHR3.0 vs eYFP first laser on epoch P < 0.0001). e Bar graph quantifying data from (d) showing that eNpHR3.0 mice have a significant laser-evoked increase in grooming compared to eYFP mice (unpaired two-tailed t test P = 0.0083). f graphical representation of behavior-dependent inhibition experiment. g Mice expressing eNpHR3.0 (N = 18) show an average increase in the duration of grooming epochs when the laser is turned on during grooming that is not seen in eYFP-WT mice (N = 10) (two-way RM ANOVA: interaction P = 0.0043, laser P = 0.0526, virus P > 0.5, subject P = 0.0010; Sidak’s multiple comparisons: eNpHR3.0 Off vs eNpHR3.0 On P = 0.0004). Black lines represent individual animals. Lines start and stop at individual animal’s average grooming event duration during laser off and on conditions. h Laser inhibition of dmPFC-DMS projections significantly extends the average duration of grooming epochs in NpHR-WT mice (N = 18) compared to eYFP-WT mice (N = 10) (unpaired two-tailed t-test P = 0.0043). Bar and line graphs show data means +/− SEM. Source data are provided as a Source data file. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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