Fig. 3: Regional biochemical differences of the mouse brain.

a Principal component analysis (PCA) of all mouse brain metabolome samples. PCA vector 1 separates samples into different brain regions. Samples are colored by brain regions. b Principal component analysis (PCA) focused on early adult mice for all 10 brain regions. PCA vector 1 scparates cerebrum and brainstem, vector 2 distinguishes the cerebellum from cerebrum and brainstem samples. Samples are colored by brain regions. c Heatmap matrix of pairwise Spearman correlations between brain regions in early adults. Strong correlations are given in red, strong negative correlations in blue. Overall correlation structures distinguish the three main brain divisions cerebrum, brainstem, and cerebellum. d Heatmap of metabolites differentially expressed across the different brain regions, constrained to metabolites with >2-fold changes. Metabolites are categorized by ClassyFire. From left to right: Benzenoids: red, Lipids: orange, Nucleosides: light green, Acids: dark blue, Nitrogen organics: purple, Oxygen organics: dark green, Heterocyclics: light blue, Others: dark gray. e Co-localization maps of dopamine metabolites and in situ hybridization of dopamine receptors. Drd1 and Drd2 in situ hybridization images are taken from the 2004 Allen Institute for Brain Science (http://mouse.brain-map.org). Image credit: Allen Institute. f Co-localization maps of adenosine metabolites and in situ hybridization of adenosine receptors Adora2a and cAMP hydrolase PED10. The Adora2a and PED10 in situ hybridization images are taken from the 2004 Allen Institute for Brain Science (http://mouse.brain-map.org). Image credit: Allen Institute. g Co-localization maps of guanine and in situ hybridization of the guanine nucleotide dissociation inhibitor Pcp2. The Pcp2 in situ hybridization image is taken from the 2004 Allen Institute for Brain Science (http://mouse.brain-map.org). Image credit: Allen Institute.