Fig. 4: Analysis of pre-60S export events.
From: Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time
a Distribution of dwell times at the pore. The bar height indicates the frequency of the observation of the respective dwell times. The blue line indicates the best fit of the probability distribution using an exponential decay model with one translocating species yielding an export duration of τ = 24 ± 4 ms. The total number of evaluated trajectories was n = 78. b Superposition of all export trajectories aligned to the transporting NPC. (Top) The trajectories were aligned to the transporting NPC (as indicated by the maximum of the eGFP-NTF2 signal) as the origin and then rotated such that the export direction coincided with the positive x axis. (Bottom) The number of pre-60S particle positions in a y axis region of ±100 nm was plotted in dependence on the location with regard to the average NTF2 accumulation. The maximum occurred at the position of the cytoplasmic ring of the NPC (red lines). The total number of single positions shown is n = 589.
